EFFICACY OF HOMEMADE APPLE PRESERVATION METHODS AND THEIR RELATIONSHIP WITH MICROBIAL CONTAMINATION
DOI:
https://doi.org/10.56238/ramv20n15-018Keywords:
Microbial Contamination, Food Preservation, Enzymatic Browning, Ascorbic AcidAbstract
This study analyzed the effectiveness of domestic preservation methods (lemon juice and ascorbic acid) on cut apples, aiming to determine the relationship between enzymatic browning and microbiological contamination. The methodology involved applying the treatments to Gala apples, which were stored under different temperature conditions (freezing, refrigeration, and ambient). Deterioration was assessed using visual oxidation scores and fungal and yeast counts. The results indicated that temperature is the most critical factor. Under refrigeration, lemon juice proved to be the most effective treatment against oxidation and contamination. Interestingly, the sample treated with ascorbic acid, despite exhibiting less browning than the control, was the most contaminated. The research concluded that enzymatic browning and microbiological contamination are independent phenomena, with visual appearance being an unreliable indicator of the fruit's microbiological safety. This finding suggests the need to consider multiple parameters for a complete assessment of food quality.
References
[1] MARTÍNEZ, M. V.; WHITAKER, J. R. The biochemistry and control of enzymatic browning. Trends in Food Science & Technology, Amsterdam, v. 6, n. 6, p. 195-200, jun. 1995. Disponível em: https://belinrae.inrae.fr/doc_num.php?explnum_id=5172 . Acesso em: 30 jul. 2025.
[2] YORUK, R.; MARSHALL, M. R. Physicochemical properties and function of plant polyphenol oxidase: a review. Food Reviews International, New York, v. 19, n. 4, p. 361-422, 2003. Disponível em: https://college.agrilife.org/talcottlab/wp-content/uploads/sites/108/2019/01/Review-PPO.pdf. Acesso em: 30 jul. 2025.
[3] SON, S. M.; MOON, K. D.; LEE, C. Y. Inhibitory effects of various antibrowning agents on apple slices. Food Chemistry, London, v. 73, n. 1, p. 23-30, abr. 2001. Disponível em: https://2024.sci-hub.se/460/cc6c222bfd63a454b90106c26a1731dd/10.1016@s0308-81460000274-0.pdf . Acesso em: 23 jul. 2025.
[4] SOLIVA-FORTUNY, R. C.; MARTÍN-BELLOSO, O. New advances in extending the shelf-life of fresh-cut fruits: a review. Trends in Food Science & Technology, Amsterdam, v. 14, n. 9, p. 341-353, set. 2003. Disponível em: https://pt.scribd.com/document/30224904/2003-Review-New-Advances-in-Extending-the-Shelf-life-of-Fresh-cut-Fruits. Acesso em: 23 jul. 2025.
[5] BOX, G. E. P.; HUNTER, J. S.; HUNTER, W. G. Statistics for experimenters: design, innovation, and discovery. 2. ed. Hoboken: Wiley-Interscience, 2005. Disponível em: https://pages.stat.wisc.edu/~yxu/Teaching/16%20spring%20Stat602/%5BGeorge_E._P._Box,_J._Stuart_Hunter,_William_G._Hu(BookZZ.org).pdf . Acesso em: 10 jul. 2025.
[6] MEILGAARD, M. C.; CIVILLE, G. V.; CARR, B. T. Sensory evaluation techniques. 5. ed. Boca Raton: CRC Press, 2016. Disponível em: https://api.pageplace.de/preview/DT0400.9781482216912_A37870469/preview-9781482216912_A37870469.pdf . Acesso em: 10 jul. 2025.
[7] JAY, J. M.; LOESSNER, M. J.; GOLDEN, D. A. Modern food microbiology. 7. ed. New York: Springer, 2005. Disponível em: https://muhammadsubchi.wordpress.com/wp-content/uploads/2010/04/modern-food-microbiology-7th-ed-springer-2005.pdf Acesso em: 10 jul. 2025.
[8] TOURNAS, V. et al. BAM Chapter 18: Yeasts, Molds, and Mycotoxins. In: U.S. FOOD AND DRUG ADMINISTRATION (FDA). Bacteriological Analytical Manual (BAM). Silver Spring: FDA, 2023. Disponível em: https://www.fda.gov/food/laboratory-methods-food/bam-chapter-18-yeasts-molds-and-mycotoxins. Acesso em: 10 jul. 2025.
[9] SON, S. M.; MOON, K. D.; LEE, C. Y. Inhibitory effects of various antibrowning agents on apple slices. Food Chemistry, London, v. 73, n. 1, p. 23-30, abr. 2001. Disponível em: https://2024.sci-hub.se/460/cc6c222bfd63a454b90106c26a1731dd/10.1016@s0308-81460000274%E2%80%930.pdf. Acesso em: 6 ago. 2025.
[10] RICO, D. et al. Extending and measuring the quality of fresh-cut fruit and vegetables: a review. Journal of the Science of Food and Agriculture, London, v. 87, n. 12, p. 2215-2234, 2007. Disponível em: https://arrow.tudublin.ie/cgi/viewcontent.cgi?article=1027&context=schfsehart. Acesso em: 6 ago. 2025.
[11] SOLIVA-FORTUNY, R. C.; MARTÍN-BELLOSO, O. New advances in extending the shelf-life of fresh-cut fruits: a review. Trends in Food Science & Technology, Amsterdam, v. 14, n. 9, p. 341-353, set. 2003. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0924224403000542. Acesso em: 6 ago. 2025.